Journal: International Journal of Cell Biology

Article Title: Changes in the Distribution of the α 3 Na + /K + ATPase Subunit in Heterozygous Lurcher Purkinje Cells as a Genetic Model of Chronic Depolarization during Development

doi: 10.1155/2014/152645

Figure Lengend Snippet: Confocal images of immunohistochemistry for activated caspase-3 (red), the α 3 isoform (green), and DAPI (blue) in +/ Lc cerebellar sections at P15. The white arrows in (a) and (b) indicate degenerating +/ Lc Purkinje cells that are also immunolabeled for the α 3 isoform. The red arrowheads indicate what appear to be the degenerating dendrites of the +/ Lc Purkinje cell surrounded by α 3 immunolabeling. The white arrowheads indicate the cell bodies of +/ Lc Purkinje cells that have not yet started to express activated caspase-3. The nuclear DAPI labeling is faint and diffuse in the neighboring +/ Lc Purkinje cells that do not yet express activated caspase-3. The monoclonal mouse anti- α 3 isoform specific only for the full 110 kD α 3 isoform was used in this case and a similar labeling pattern is observed compared to the rabbit polyclonal α 3 antibody. Scale bars: 20 μ m.

Article Snippet: Sections were double labeled with either rabbit polyclonal anti- α 1 (gift of Dr. M. Blaustein) or rabbit polyclonal anti- α 3 Na/K ATPase (Upstate, now Millipore, 1/500: and mouse monoclonal anti-calbindin (Sigma, 1/5000) or rabbit polyclonal anti-activated caspase-3 (R&D, 1/500) and monoclonal mouse anti- α 3 Na + /K + ATPase (ABR Affinity BioReagents, Thermo Scientific Pierce Antibodies, 1/250).

Techniques: Immunohistochemistry, Immunolabeling, Labeling